Glycolysis Regulation and Key Enzymes

  • Net glycolysis (cytoplasm): Glucose + 2 Pi + 2 ADP + 2 NAD+ → 2 pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O.
  • Equation not balanced chemically, and exact balanced equation depends on ionization state of reactants and products.
  • Require ATP
    • Glucose-6-P ⊝ hexokinase.
    • Fructose-6-P ⊝ glucokinase.
    • AMP ⊕, fructose-2,6-bisphosphate ⊕.
    • ATP ⊝, citrate ⊝.
  • Produce ATP
    • Fructose-1,6-bisphosphate ⊕
    • ATP ⊝, alanine ⊝.

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